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chicken polyclonal anti tgf β1 primary antibody  (R&D Systems)


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    R&D Systems chicken polyclonal anti tgf β1 primary antibody
    Figure 2 <t>TGF-β1</t> steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.
    Chicken Polyclonal Anti Tgf β1 Primary Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Transforming growth factor-β1/Smad3-independent epithelial–mesenchymal transition in type I collagen glomerulopathy"

    Article Title: Transforming growth factor-β1/Smad3-independent epithelial–mesenchymal transition in type I collagen glomerulopathy

    Journal: International Journal of Nephrology and Renovascular Disease

    doi: 10.2147/ijnrd.s141393

    Figure 2 TGF-β1 steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.
    Figure Legend Snippet: Figure 2 TGF-β1 steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.

    Techniques Used: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Polymerase Chain Reaction



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    Figure 2 <t>TGF-β1</t> steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.
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    Figure 2 <t>TGF-β1</t> steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.
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    Image Search Results


    Figure 2 TGF-β1 steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.

    Journal: International Journal of Nephrology and Renovascular Disease

    Article Title: Transforming growth factor-β1/Smad3-independent epithelial–mesenchymal transition in type I collagen glomerulopathy

    doi: 10.2147/ijnrd.s141393

    Figure Lengend Snippet: Figure 2 TGF-β1 steady-state mRNA and protein are not upregulated in homotrimeric type I collagen glomerulopathy. Notes: (A) Quantitative RT-PCR demonstrates that TGF-β1 copy number is not significantly different in glomerular isolates of 1-month-old wildtype (+/+) and Col1a2-deficient (−/−) mice. However, by 3 months of age the TGF-β1 copy number is reduced in Col1a2-deficient glomerular isolates as compared to wildtype glomerular isolates (*p≤ 0.05). (B) Although protein immunoassay demonstrated no significant difference in picograms of TGF-β1 protein per glomerulus in wildtype (+/+) and Col1a2-deficient (−/−) glomeruli at both 1 month and 3 months of age, Col1a2-deficient (−/−) glomeruli at 1 month of age exhibited a greater variability in TGF-β1 protein per glomerulus. Abbreviations: TGF-β1, tumor growth factor β1; RT-PCR, reverse transcriptase- polymerase chain reaction.

    Article Snippet: TGFβ-R1 IHC using 1) rabbit polyclonal anti-TGFβ-RI primary antibody at 1:50 (sc-398; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 2) chicken polyclonal anti-TGF-β1 primary antibody at 1:100 (AB-101-NA; R & D Systems, Minneapolis, MN, In te rn at io na l J ou rn al o f N ep hr ol og y an d R en ov as cu la r D is ea se d ow nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 18 8.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Polymerase Chain Reaction

    (A) Representative phase-contrast images of BALB/c or SPRET/EiJ organoids induced to branch with TGFα in growth factor-reduced Matrigel. Organoids were grown for 5 days after treatment. (B) Quantification of the number of BALB/c and SPRET/EiJ organoids in each condition that had three or more branches (n = 6 experiments, >200 organoids/condition). (C) TGFβ1 levels after treatment with TGFα. ELISA analysis of culture media harvested from organoid cultures (n = 6 independent experimental sets). (D) Merged channel images BALB/c and SPRET/EiJ mammary gland sections stained with DAPI, latent-TGFβ1 and active-TGFβ1 (n = 6 experiments). (E) Representative phase-contrast images of BALB/c organoids induced to branch with TGFα. After 24 hours, organoids were treated with SPRET/EiJ culture media either alone or with TGFβ1 blocking antibody and grown for 5 days. Quantification of the number of BALB/c organoids in each condition that had three or more branches (n = 6 experiments, >100 organoids/condition). The p-values were obtained by t-test.

    Journal: Scientific Reports

    Article Title: Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment

    doi: 10.1038/srep08919

    Figure Lengend Snippet: (A) Representative phase-contrast images of BALB/c or SPRET/EiJ organoids induced to branch with TGFα in growth factor-reduced Matrigel. Organoids were grown for 5 days after treatment. (B) Quantification of the number of BALB/c and SPRET/EiJ organoids in each condition that had three or more branches (n = 6 experiments, >200 organoids/condition). (C) TGFβ1 levels after treatment with TGFα. ELISA analysis of culture media harvested from organoid cultures (n = 6 independent experimental sets). (D) Merged channel images BALB/c and SPRET/EiJ mammary gland sections stained with DAPI, latent-TGFβ1 and active-TGFβ1 (n = 6 experiments). (E) Representative phase-contrast images of BALB/c organoids induced to branch with TGFα. After 24 hours, organoids were treated with SPRET/EiJ culture media either alone or with TGFβ1 blocking antibody and grown for 5 days. Quantification of the number of BALB/c organoids in each condition that had three or more branches (n = 6 experiments, >100 organoids/condition). The p-values were obtained by t-test.

    Article Snippet: Slides were incubated overnight with either a polyclonal chicken antibody against active TGFβ1 antibody (R&D Systems AF-101-NA, 10) or a polyclonal goat antibody against LAP TGFβ1 (R&D Systems AB-246-NA, 50).

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Blocking Assay